METHOD FOR DETERMINING optimum concentration of substances of cryoprotective medium at the freezing of semen RUSSIAN STURGEON

摘要

1.method u043eu043fu0440u0435u0434u0435u043bu0435u043du0438u00a0 optimal concentrations of substances u0441u043eu0441u0442u0430u0432u043bu00a0u044eu0449u0438u0445 u043au0440u0438u043eu043fu0440u043eu0442u0435u043au0442u043eu0440u043du0443u044e environment for freezing semen of russian sturgeon and u043cu043eu0440u0444u043eu0444u0443u043du043au0446u0438u043eu043du0430 u043bu044cu043du0430u00a0 quality assessment of germ cells, including the use of glycerol and dhs u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0, further as the u0430u043du0442u0438u043au043eu0430u0433u0443u043bu00a0u043du0442u0430 in cryogenic environments i u0432u043du043eu0441u00a0u0442 heparin.and after the tread u0432u043du0435u0441u0435u043du0438u00a0 concentration in semen u043eu043fu0440u0435u0434u0435u043bu00a0u044eu0442 motility u0441u043fu0435u0440u043cu0438u0435u0432 and u043fu0440u043eu0432u043eu0434u00a0u0442 microscopic analysis u0434u043bu00a0 u0432u044bu00a0u0432u043bu0435u043du0438u00a0 negative sign of sexual cells, then u043eu0441u0443u0449u0435u0441u0442u0432u043bu00a0u044eu0442 u043cu0438u043au0440u043eu0441u043au043eu043fu0438u0440u043eu0432u0430u043du0438u0435 cells on the smear of seminal fluid, u043eu043au0440u0430u0448u0435u043du043du043eu043c h & + u044du043eu0437u0438u043du043eu043c and compare there u0441u043fu0435u0440u043cu0438u0435u0432 quality control (u043du0430u0442u0438u0432u043du0430u00a0 u0441u0435u043cu0435u043du043du0430u00a0 liquid without tread)and after that all the substances in the u043au043eu043du0446u0435u043du0442u0440u0430u0446u0438u00a0u0445, non-toxic u0434u043bu00a0 cells, mix 1: 1: 1, 0.5 ml of liquid.;2. method u043eu043fu0440u0435u0434u0435u043bu0435u043du0438u00a0 optimal concentrations of substances u0441u043eu0441u0442u0430u0432u043bu00a0u044eu0449u0438u0445 u043au0440u0438u043eu043fu0440u043eu0442u0435u043au0442u043eu0440u043du0443u044e environment for freezing semen of russian sturgeon, u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0 order that u0434u043bu00a0 accurately u043eu043fu0440u0435u0434u0435u043bu0435u043du0438u00a0 safe concentrations of substances u0441u043eu0441u0442u0430u0432u043bu00a0u044eu0442 scale u0434u043bu00a0 cryoprotectants concentrations of each substance in the arithmetic or u0433u0435u043eu043cu0435u0442u0440u0438 on the u043fu0440u043eu0433u0440u0435u0441u0441u0438u00a0u0445.