首页>
外国专利>
STANDARDIZED TESTS FOR SENSITIVITY FOR AEROBIC PATHOGENS
STANDARDIZED TESTS FOR SENSITIVITY FOR AEROBIC PATHOGENS
展开▼
机译:需氧致病菌敏感性的标准测试
展开▼
页面导航
摘要
著录项
相似文献
摘要
1. the environment u0434u043bu00a0 u043fu0440u043eu0432u0435u0434u0435u043du0438u00a0 analysis on sensitivity of organisms u0434u043bu00a0 u043au043eu043du0442u0440u043eu043bu00a0 american collection of model crops (u0430u0442u0441u0441) know u0449u0438u0439u0441u00a0 because that the environment contains a nutrient medium, antibiotic and immunologic adjuvant in sufficient quantity u0434u043bu00a0 order to stabilize analysis u0447u0443u0432u0441u0442u0432u0438u0442 u0435u043bu044cu043du043eu0441u0442u0438 and improve its quality.;2. the environment 1, u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0 as immunologic adjuvant select from group u0446u0438u0441u0442u0435u0438u043du0430, u0442u0438u043eu0433u043bu0438u043au043eu043bu0430u0442u0430, u0430u0441u043au043eu0440u0431u0438u043du043eu0432u043eu0439 acid, u043fu0438u0440u0443u0432u0430u0442u0430 and u043au0430u0442u0430u043bu0430u0437u044b.;3. the environment 1, u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0 as immunologic adjuvant derived from u0446u0438u0442u043eu043fu043bu0430u0437u043cu0430u0442u0438u0447u0435u0441u043au0438u0445 membranes of microorganisms.;4. the environment for 2, u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0 as immunologic adjuvant is present in the range of concentrations of approximately 00005 to about 5.0% mas. / on. the whole environment.;5. the environment for p.4, u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0 as immunologic adjuvant is present in the range of from about 0.005 to about 0.5% mas. / on.;6. the environment for p.4, u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0 as immunologic adjuvant is present in concentrations of about 0.05% mas. / on.;7. the environment 3, u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0 as immunologic adjuvant is present in quantities of about 0.5% to about 10.0%. / on. the whole environment.;8. the environment 3, u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0 as immunologic adjuvant is present in amount from about 1.0 to about 4.0% (of / about).;9. the environment 3, u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0 as immunologic adjuvant is of about 2.0% (of / about).;10. the environment in any of the u043fu043f.1 - 9, u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0, u043fu0440u0435u0434u0441u0442u0430u0432u043bu00a0u0435u0442 a tetracycline antibiotic, e.g., 7, 9 u0437u0430u043cu0435u0449u0435u043du043du044bu0439 tetracycline.;11. the environment art.u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0 9, so that u0442u0435u0442u0440u0430u0446u0438u043au043bu0438u043du043eu0432u044bu0439 antibiotic choice of tetracycline (tetracycline), u0445u043bu043eu0440u0442u0435u0442u0440u0430u0446u0438u043au043bu0438u043du0430 (chlortetracycline), u043eu043au0441u0438u0442u0435u0442u0440u0430u0446u0438u043au043bu0438u043du0430 (oxytetracycline), u0434u0438u043cu0435u0442u0438u043bu0445u043bu043eu0440 tetracycline (dimethylchlortetracycline), u0434u0435u043cu0435u043au043bu043eu0446u0438u043au043bu0438u043du0430 (demeclocycline), u043cu0435u0442u0430u0446u0438u043au043bu0438u043du0430 (methacycline), u043bu0438u043cu0435u0446u0438u043au043bu0438u043du0430 (lymecycline), u043au043bu043eu043cu043eu0446u0438u043au043bu0438u043du0430 (clomocycline), u0434u043eu043au0441u0438u0446u0438u043au043bu0438u043du0430 (doxycycline), u043cu0438u043du043eu0446u0438u043au043bu0438u043du0430 (minocycline)n, n - u0434u0438u043cu0435u0442u0438u043bu0433u043bu0438u0446u0438u043bu0430u043cu0438u0434u043e 9 u0430u043cu0438u043du043eu043cu0438u043du043eu0446u0438u043au043bu0438u043du0430 (dmg - mino), n, n - u0434u0438u043cu0435u0442u0438u043bu0433u043bu0438u0446u0438u043bu0430u043cu0438u0434u043e 9 amino - 6 - u0434u0435u043cu0435u0442u0438u043b - 6 - u0434u0435u043eu043au0441u0438u0442u0435u0442u0440u0430u0446u0438u043au043bu0438u043du0430 (dmg - DMDOT) and u0442u0438u0433u0435u0446u0438u043au043bu0438u043du0430 (tigecycline).;12. the environment in any of the u043fu043f.3 - 9, 11, u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0 because microorganisms chosen from the group Escheiichia coli, Salmonella typhimurium, Gluconobacter Pseudomonas oxydans and pseudomonas aeruginosa.;13. method u0441u0442u0430u0431u0438u043bu0438u0437u0438u0440u043eu0432u0430u043du0438u00a0 values of minimum u0438u043du0433u0438u0431u0438u0440u0443u044eu0449u0435u0439 concentration (mic) generated by the test u0434u043bu00a0 organisms u0434u043bu00a0 u043au043eu043du0442u0440u043eu043bu00a0 quality from the united states in the collection of model crops (u0430u0442u0441u0441), comprising the following steps:;as well. addendum to the environment u0430u0434u044au044eu0432u0430u043du0442u0430, sufficient u0434u043bu00a0 order to stabilize sensitivity analysis and quality, and an antibiotic, u043eu0431u0440u0430u0437u0443u044eu0449u0438 x environment u0434u043bu00a0 analysis;;the enclosure test organism in the environment u0434u043bu00a0 analysis;;with the. the environment u0438u043du043au0443u0431u0438u0440u043eu0432u0430u043du0438u0435 u0434u043bu00a0 analysis;;the definition of u0437u043du0430u0447u0435u043du0438u00a0 mick.;14. method u0441u0442u0430u0431u0438u043bu0438u0437u0438u0440u043eu0432u0430u043du0438u00a0 u0437u043du0430u0447u0435u043du0438u00a0 minimum u0438u043du0433u0438u0431u0438u0440u0443u044eu0449u0435u0439 concentration (mic) generated by the test u0434u043bu00a0 organisms u0434u043bu00a0 u043au043eu043du0442u0440u043eu043bu00a0 quality from the united states in the collection of model crops (u0430u0442u0441u0441), comprising the following steps:;as well. preparation of environment based on the mueller hinton broth - ii by u0434u043eu0431u0430u0432u043bu0435u043du0438u00a0 22 g powder environment to 1.0 litres of distilled water;;(b) u0430u0432u0442u043eu043au043bu0430u0432u0438u0440u043eu0432u0430u043du0438u0435 (121u0441, 15 pound / u043au0432.u0434u044eu0439u043c, 15 min) and the cooling environment.;with the. appendix Oxyrase in concentrations up to 10% of the final volume and soak in the 35 - 37u0441 in an incubator for 30 min;;the addition of approximately 50 mkl [environment] prepared in each well of 96 u043bu0443u043du043eu0447u043du043eu0433u043e u043cu0438u043au0440u043eu0442u0438u0442u0440u0430u0446u0438u043eu043du043du043eu0433u043e tablet;;e. the weighting of an antibiotic and an addendum to the medicinal broth u0434u043bu00a0 u0434u043eu0432u0435u0434u0435u043du0438u00a0 to concentrations approximately (usually 128 mg / ml).;f enclosure 50 mkl antibiotics in the first u0440u00a0u0434 holes u0442u0438u0442u0440u0430u0446u0438u043eu043du043du043eu0433u043e tablet;;g. breeding u0434u0432u0443u043au0440u0430u0442u043du044bu043cu0438 serial u0440u0430u0437u0432u0435u0434u0435u043du0438u00a0u043cu0438 across or along the board.;h preparation u0438u043du043eu043au0443u043bu00a0u0442u0430 prompt in a concentration of 108 / ml;;the breeding u0438u043du043eu043au0443u043bu00a0u0442u0430 1: 100 in the broth in a concentration of 10 mkl in 9.9 ml (1, 100) u0441u043eu0441u0442u0430u0432u043bu00a0u0435u0442 106 u043au043eu043bu043eu043du0438u0435u043eu0431u0440u0430u0437u0443u044eu0449u0438u0445 units (a), the standardized u0438u043du043eu043au0443u043bu00a0u0442;;j enclosure 50 mkl standardized u0438u043du043eu043au0443u043bu00a0u0442u0430 in holes u043cu0438u043au0440u043eu0442u0438u0442u0440u0430u0446u0438u043eu043du043du043eu0433u043e tablet;;k. u0438u043du043au0443u0431u0438u0440u043eu0432u0430u043du0438u0435 and in 35 37u0441 for 18 to 22 h; and;(i) the definition of mick to the naked eye.;15. method for u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0 p.13 or 14, so that the immunologic adjuvant derived from u0446u0438u0442u043eu043fu043bu0430u0437u043cu0430u0442u0438u0447u0435u0441u043au0438u0445 membranes of microorganisms.;16. method for u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0 p.15, as immunologic adjuvant is present in quantities of about 0.5% to about 10.0%. / on. the whole environment.;17. method for u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0 p.15, as immunologic adjuvant is present in the environment of from about 1.0% to about 4.0% (of / about).;18. method for u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0 p.15, as immunologic adjuvant is of about 2.0% (of / about).;19. way on any of the u043fu043f.13, 14, 16, 18, u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0, u043fu0440u0435u0434u0441u0442u0430u0432u043bu00a0u0435u0442 a tetracycline antibiotic, for example, 7.9 u0437u0430u043cu0435u0449u0435u043du043du044bu0439 tetracycline.;20. method for p.u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0 19, so that the antibiotic chosen from groups of tetracycline (tetracycline), u0445u043bu043eu0440u0442u0435u0442u0440u0430u0446u0438u043au043bu0438u043du0430 (chlortetracycline), u043eu043au0441u0438u0442u0435u0442u0440u0430u0446u0438u043au043bu0438u043du0430 (oxytetracycline), u0434u0438u043cu0435u0442u0438u043bu0445u043bu043eu0440u0442u0435u0442u0440u0430u0446u0438u043au043bu0438u043du0430 (dime thylchlortetracycline), u0434u0435u043cu0435u043au043bu043eu0446u0438u043au043bu0438u043du0430 (demeclocycline), u043cu0435u0442u0430u0446u0438u043au043bu0438u043du0430 (methacycline), u043bu0438u043cu0435u0446u0438u043au043bu0438u043du0430 (lymecycline), u043au043bu043eu043cu043eu0446u0438u043au043bu0438u043du0430 (clomocycline), u0434u043eu043au0441u0438u0446u0438u043au043bu0438u043du0430 (doxycycline), u043cu0438u043du043eu0446u0438u043au043bu0438u043du0430 (minocycline), nn - u0434u0438u043cu0435u0442u0438u043bu0433u043bu0438u0446u0438u043bu0430u043cu0438u0434u043e 9 u0430u043cu0438u043du043eu043cu0438u043du043eu0446u0438u043au043bu0438u043du0430 (dmg - mino), n, n - u0434u0438u043cu0435u0442u0438u043bu0433u043bu0438u0446u0438u043bu0430u043cu0438u0434u043e 9 amino - 6 - u0434u0435u043cu0435u0442u0438u043b - 6 - u0434u0435u043eu043au0441u0438u0442u0435u0442u0440u0430u0446u0438u043au043bu0438u043du0430 (dmg - DMDOT) and u0442u0438u0433u0435u0446u0438u043au043bu0438u043du0430 (tigecycline).;21. way on any of the u043fu043f.16 - 18 or 20, u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0 because microorganisms chosen from the group: Escheiichia coli. Salmonella typhimurium, Gluconobacter Pseudomonas oxydans and pseudomonas aeruginosa.;22.the environment u0434u043bu00a0 u043fu0440u043eu0432u0435u0434u0435u043bu0435u043du0438u00a0 sensitivity analysis u0434u043bu00a0 organisms u0434u043bu00a0 u043au043eu043du0442u0440u043eu043bu00a0 quality from the american collection of model crops (u0430u0442u0441u0441), u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0, h then the composition of the environment provides a permissive environment, immunologic adjuvant and an antibiotic, and the immunologic adjuvant is present in sufficient quantity u0434u043bu00a0 order under u0430u0432u0438u0442u044c building u0440u0430u043du043du0435u0433u043eu00bb peaku043eu043fu0440u0435u0434u0435u043bu00a0u0435u043cu043eu0433u043e liquid u0445u0440u043eu043cu0430u0442u043eu0433u0440u0430u0444u0438u0435u0439 high u0434u0430u0432u043bu0435u043du0438u00a0 in time u0443u0434u0435u0440u0436u0438u0432u0430u043du0438u00a0 approximately 11.5 - 12.0 min.;23. the environment, p.22, u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0 as immunologic adjuvant derived from u0446u0438u0442u043eu043fu043bu0430u0437u043cu0430u0442u0438u0447u0435u0441u043au0438u0445 membranes of microorganisms.;24. the environment on the p.23, u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0 as immunologic adjuvant is present in the environment of from about 0.5 to about 10.0%.;25. the environment u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0 p.23, immunologic adjuvant is present in the environment of from about 1.0 to about 4.0%.;26. the environment on the p.23, u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0 as immunologic adjuvant is of about 2.0%.;27. the environment in any of the u043fu043f.22 - 26, u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0, u043fu0440u0435u0434u0441u0442u0430u0432u043bu00a0u0435u0442 a tetracycline antibiotic, such as tetracycline u0437u0430u043cu0435u0449u0435u043du043du044bu0439 7.9.;28. the environment art.27 u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0, antibiotic choice of tetracycline (tetracycline), u0445u043bu043eu0440u0442u0435u0442u0440u0430u0446u0438u043au043bu0438u043du0430 (chlortetracycline), u043eu043au0441u0438u0442u0435u0442u0440u0430u0446u0438u043au043bu0438u043du0430 (oxytetracycline), u0434u0438u043cu0435u0442u0438u043bu0445u043bu043eu0440u0442u0435u0442u0440u0430u0446u0438u043au043bu0438u043du0430 (dimet hylchlortetracycline), u0434u0435u043cu0435u043au043bu043eu0446u0438u043au043bu0438u043du0430 (demeclocycline), u043cu0435u0442u0430u0446u0438u043au043bu0438u043du0430 (methacycline), u043bu0438u043cu0435u0446u0438u043au043bu0438u043du0430 (lymecycline), u043au043bu043eu043cu043eu0446u0438u043au043bu0438u043du0430 (clomocycline), u0434u043eu043au0441u0438u0446u0438u043au043bu0438u043du0430 (doxycycline), u043cu0438u043du043eu0446u0438u043au043bu0438u043du0430 (minocycline), nn - u0434u0438u043cu0435u0442u0438u043bu0433u043bu0438u0446u0438u043bu0430u043cu0438u0434u043e 9 u0430u043cu0438u043du043eu043cu0438u043du043eu0446u0438u043au043bu0438u043du0430 (dmg - mino), n, n - u0434u0438u043cu0435u0442u0438u043bu0433u043bu0438u0446u0438u043bu0430u043cu0438u0434u043e 9 amino - 6 - u0434u0435u043cu0435u0442u0438u043b - 6 - u0434u0435u043eu043au0441u0438u0442u0435u0442u0440u0430u0446u0438u043au043bu0438u043du0430 (dmg - DMDOT) and u0442u0438u0433u0435u0446u0438u043au043bu0438u043du0430 (tigecycline).;29. the environment, p.27, u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0 because microorganisms chosen from the group Salmonella typhimurium, escherichia coli, pseudomonas aeruginosa and Gluconobacter oxydans Pseudomonas.;30.the environment u0434u043bu00a0 u043fu0440u043eu0432u0435u0434u0435u043du0438u00a0 sensitivity analysis u0434u043bu00a0 organisms u0434u043bu00a0 u043au043eu043du0442u0440u043eu043bu00a0 quality from the american collection of model crops (u0430u0442u0441u0441), u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0 what the composition of the environment provides a permissive environment, immunologic adjuvant and u0442u0435u0442u0440u0430u0446u0438u043au043bu0438u043du043eu0432u044bu0439 antibiotic, and the immunologic adjuvant is present in sufficient quantity u0434u043bu00a0 in order to suppress the formation of the "u0440u0430u043du043du0435u0433u043eu00bb peaku043eu043fu0440u0435u0434u0435u043bu00a0u0435u043cu043eu0433u043e liquid u0445u0440u043eu043cu0430u0442u043eu0433u0440u0430u0444u0438u0435u0439 high u0434u0430u0432u043bu0435u043du0438u00a0.;31.the environment u0434u043bu00a0 u043eu043fu0440u0435u0434u0435u043bu0435u043du0438u00a0 sensitivity analysis u0434u043bu00a0 organisms u0434u043bu00a0 u043au043eu043du0442u0440u043eu043bu00a0 quality from the american collection of model crops (u0430u0442u0441u0441), u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0 those wh on the composition of the environment provides a permissive environment, immunologic adjuvant and 7.9 u0437u0430u043cu0435u0449u0435u043du043du044bu0439 u0442u0435u0442u0440u0430u0446u0438u043au043bu0438u043du043eu0432u044bu0439 antibiotic, and the immunologic adjuvant is present in quantities up to u0441u0442u0430u0442u043eu0447u043du043eu043c u0434u043bu00a0 u043fu043eu0434u0430u0432u043bu0435u043du0438u00a0 u0444u043eu0440u043cu0438u0440u043eu0432u0430u043du0438u00a0 "u0440u0430u043du043du0435u0433u043eu00bb peaku043eu043fu0440u0435u0434u0435u043bu00a0u0435u043cu043eu0433u043e liquid u0445u0440u043eu043cu0430u0442u043eu0433u0440u0430u0444u0438u0435u0439 high u0434u0430u0432u043bu0435u043du0438u00a0.;32. the environment u0434u043bu00a0 u043eu043fu0440u0435u0434u0435u043bu0435u043du0438u00a0 sensitivity analysis u0434u043bu00a0 organisms u0434u043bu00a0 u043au043eu043du0442u0440u043eu043bu00a0 quality from the american collection of model crops (u0430u0442u0441u0441), u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0 those wh on the composition of the environment contains fresh nutrient medium and the antibiotic u0434u043bu00a0 order to stabilize sensitivity analysis and improve its quality.;33. the environment u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0 p.32, so that the composition of the environment provides a permissive environment, immunologic adjuvant and antibiotic - u0442u0438u0433u0435u0446u0438u043au043bu0438u043d, with the u043fu0440u0438u0441u0443u0442u0441 immunologic adjuvant u0442u0432u0443u0435u0442 sufficient u0434u043bu00a0, to suppress the formation of the "u0440u0430u043du043du0435u0433u043eu00bb peak, u043eu043fu0440u0435u0434u0435u043bu00a0u0435u043cu043eu0433u043e liquid u0445u0440u043eu043cu0430u0442u043eu0433u0440u0430u0444u0438u0435u0439 high u0434u0430u0432u043bu0435u043du0438u00a0 in time subject u0436u0438u0432u0430u043du0438u00a0 approximately 11.5 - 12.0 min.;34. the environment, p.32, u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0, u043fu0438u0442u0430u0442u0435u043bu044cu043du0430u00a0 environment is made not more than 12 hours ago.;35. the environment, p.32, u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0, antibiotic u043fu0440u0435u0434u0441u0442u0430u0432u043bu00a0u0435u0442 a u0442u0438u0433u0435u0446u0438u043au043bu0438u043d (Tigecycline).
展开▼