METHOD OF ASSESSMENT pharmacological agents at the local protection factor humans at CASE patients bronchopulmonary diseases

摘要

A method of evaluating the action of pharmacological preparations for local human body protection factors for example patients bronchopulmonary diseases, comprising the selection of the test material, obtaining therefrom a cell slurry, determination of the enzymatic activity of phagocytes before and after exposure to human pharmacological drug calculation index reserve possibilities cells, their comparison between and an assessment of pharmacological action of the drug on the comparison results, characterized in that the selection of the test material and carried from induced human sputum, determining the enzymatic activity of phagocytes is carried out for at least 5 parameters, wherein further comprising determining the enzymatic activity of phagocytes in healthy people, reserve cell capacity indicators are calculated according to the formula: OPRopyt - optical cell density solution stimulated by bacteria, OPRiskh - unstimulated cells (OD × 10), PRV - figure reserve capacity of cells; thus reserve capacity of cells derived parameters further comparing out with the indicators of reserve capacity of cells in healthy people and their changes are judged on the pharmacological action of the drug on the local protection cheloveka.2 organism factors. A method according to claim 1, characterized in that the enzymatic activity of phagocytes is determined spectrophotometrically.