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APPLICATION OF THE MATRIX OF REVERSE GENETIC ENGINEERING FOR OBTAINING PROTEIN VACCINES AND PROTEIN VACCINE OF AVIAN INFLUENZA VIRUS
APPLICATION OF THE MATRIX OF REVERSE GENETIC ENGINEERING FOR OBTAINING PROTEIN VACCINES AND PROTEIN VACCINE OF AVIAN INFLUENZA VIRUS
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机译:反向遗传工程矩阵在获得禽流感病毒蛋白疫苗和蛋白疫苗中的应用
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摘要
The present invention relates to a preparation method of protein vaccines, and comprises the steps of: (a) providing at least one amino acid sequence of an epitope of a target antigen protein; (b) converting the amino acid sequence into a nucleic acid sequence and modifying the codons; (c) synthesising a plurality of primers of the modified nucleic acid sequence; (d) synthesising the modified nucleic acid sequence in vitro; (e) inserting the synthesized fragment of the modified nucleic acid sequence into a plasmid; (f) transforming the plasmid into a host cell to produce the modified nucleic acid encoded epitope peptide; and (g) collecting and purifying the produced peptide.
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