USING A REVERSE GENETIC ENGINEERING PLATFORM TO PRODUCE PROTEIN VACCINES AND PROTEIN VACCINE OF AVIAN INFLUENZA VIRUS

摘要

Using a Reverse Genetic Engineering Platform to Produce Protein Vaccines and Protein Vaccine of Avian Influenza virusABSTRACT OF THE DISCLOSUREThe present invention relates to a preparation method of protein vaccines, and comprises the steps of. (a) providing at least one amino acid sequence of an epitope of a target antigen protein; (b) converting the aminoacid sequence into a nucleic acid sequence and modifying the codons; (c) synthesising a plurality of primers of the modified nucleic acid sequence; (d) synthesising the modified nucleic acid sequence in vitro; (e) inserting the synthesized fragment of the modified nucleic acid sequence into a plasmid; (f) transforming the plasmid into a host cell to produce the modified nucleic10 acid encoded epitope peptide; and (g) collecting and purifying the produced peptide.Figure 14