METHOD FOR DIAGNOSING HAND AND FOOT ONYCHOMYCOSIS LESION

摘要

FIELD: medicine.;SUBSTANCE: method involves identifying Trichophyton rubrum by applying polymerase chain reaction of DNA separated from skin or nail plate sample incubated in 2-4 M alkaline solution for at least 1 h at 50-65°C. The solution is neutralized with 30% HCl acid, the sample is resedimented in 50% ethanol solution at -10 - -20°C and dried at 50-65°C. The DNA polymerase chain reaction is carried out with synthetic DNA-probes having sequence of TR-1-F (sense): 5'atcgaatctttgaacgcaca'; TR-1-F (antisense): 5'tataaagattggggccaggg 3' highly specific for gene 28 S Trichophyton rubrum RNA. The result being positive, onychomycosis is diagnosed caused by Trichophyton rubrum fungus.;EFFECT: high specificity of diagnosis method.;2 dwg, 1 tbl