A method for quantitative determination of the colonies of molecule calibration markers in tissue sections

摘要

The invention relates to a method for quantitative determination of the colonies of at least two molecule calibration markers in tissue sections, in particular in the skin - and mucus skin tissue cuts. The method according to the invention is characterized by the following process steps:- Generation of a digitized image of the tissue section for each of the at least two labeled molecular marker to the representation of the respective molecule marker,- Superimposition of the digitized images to a sum image,- pixel-by-pixel quantization of the molecule marker signal for each of the at least two labeled molecular marker in the sum image,- Generation of a matrix, wherein each pixel of a vector is assigned, the components of the quantized molecule marker signals of the at least two are labeled molecular marker, and- tissue-specific standardized alignment of the matrix.Furthermore, the invention relates to a device for the quantitative determination of the colonies of at least two molecule calibration markers in tissue sections, in particular in the skin - and mucus skin tissue cuts, as well as the use of the process according to the invention.