A process for the isothermal amplification of nucleic acids and a method for the detection of nucleic acids by simultaneous isothermal amplification of nucleic acids and signal probe

摘要

A process for the isothermal amplification of target nucleic acids, the method comprising:(a) denaturing of a reaction mixture, the (i) of target nucleic acids, (ii) an external set of a base sequence complementary to the target nucleic acids, and (iii) an internal rna / dna - hybrid primer set, the target nucleic acids to the partially complementary base sequence; and(b) amplifying the target nucleic acids in isothermal temperature after the addition of an enzymatic reaction solution mixture, the rnase and dna - polymerase, which leads to a strand displacement of the reaction mixture is in the position in step (a) was denatured.