Method for separating vWF into high molecular weight vWF and low molecular weight vWF

摘要

Sepn. of von Willebrand factor (vWF) into high and low mol. wt. fractions comprises binding vWF to an affinity column and carrying out elution at different salt concns. Also new are: (1) low mol. wt. fraction (A) of vWF, consisting mainly of dimers and tetramers and (2) high mol. wt. fraction (B) of at 50 (pref. 60)% increased activity (per mu g. of protein) in platelet aggregation compared with the physiological mixt. of both forms.