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PARTICLE MATERIAL FOR SPINE CLEANING OF BIOMAKROLEOLS, SUCH AS PLASMID DNA, CHROMOSOME DNA, RNA, VIRAL DNA, BACTERIA AND VIRUSES
PARTICLE MATERIAL FOR SPINE CLEANING OF BIOMAKROLEOLS, SUCH AS PLASMID DNA, CHROMOSOME DNA, RNA, VIRAL DNA, BACTERIA AND VIRUSES
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机译:用于生物素分子清洁的颗粒材料,例如质粒DNA,染色体DNA,RNA,病毒DNA,细菌和病毒
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摘要
The present invention relates to particulate material having a density of at least 2.5 g/ml, where the particles of the particulate material have an average diameter of 5-75 mu m, and the particles of the particulate material are essentially constructed of a polymeric base matrix, e.g. a polysaccharide such as agarose, and a non-porous core material, e.g. steel and titanium, said core material having a density of at least 3.0 g/ml, said polymeric base matrix including pendant groups which are positively charged at pH 4.0 or which are affinity ligands for a bio-molecule. Possible pendant groups include polyethyleneimine (PEI), diethylaminoethyl (DEAE) and quaternary aminoethyl (QAE). The materials are useful in expanded bed or fluidised bed chromatography processes, in particular for purification of bio-macromolecules such as plasmid DNA, chromosomal DNA, RNA, viral DNA, bacteria and viruses.
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机译:本发明涉及具有至少2.5g / ml的密度的颗粒材料,其中颗粒材料的颗粒具有5-75μm的平均直径,并且颗粒材料的颗粒基本上由聚合物基质构成。矩阵,例如多糖如琼脂糖和无孔核心材料,例如钢和钛,所述核心材料的密度至少为3.0 g / ml,所述聚合物基础基质包括侧基,这些侧基在pH 4.0时带正电,或者是生物分子的亲和配体。可能的侧基包括聚乙烯亚胺(PEI),二乙基氨基乙基(DEAE)和季氨基乙基(QAE)。该材料可用于膨胀床或流化床色谱法中,特别是用于纯化生物大分子,例如质粒DNA,染色体DNA,RNA,病毒DNA,细菌和病毒。
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