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Method for preparing nucleic acid or DNA samples and a DNA extraction process using thermophilic proteases
Method for preparing nucleic acid or DNA samples and a DNA extraction process using thermophilic proteases
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机译:使用嗜热蛋白酶的核酸或DNA样品的制备方法和DNA提取方法
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摘要
The present invention relates to the preparation of a method for the preparation of nucleic acid samples, including the steps of adding at least one non-specific thermophilic enzyme to a sample containing nucleic acid for testing, and incubating said sample for a preferred period at 65-80° C. as required to effect one or more of lysis of cells, digestion of proteins, digestion of cell-wall enzymes, via activity of said thermophilic enzyme, wherein said thermophilic enzyme is substantially stable and active at 65-80° C. but which is readily autolysed and/or denatured when said sample is incubated at or above 90° C.
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