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procedures for the isolation and characterization of potential functions based on a biological sample that contains nucleic acid

机译:基于包含核酸的生物样品分离和鉴定潜在功能的程序

摘要

The present invention is directed to a process for the separation and characterization of the functions potentially present in a biological sample containing nucleic acids, characterized in that it comprises the steps of preparation of nucleic acid fragments starting from said sample, association of each one of said fragments with a vector molecule, isolation of each fragment associated with a vector molecule or with a part of each construction composed of a fragment associated with a vector molecule, in vitro treatment of each fragment associated with a vector molecule or of a part of each construction composed of a fragment associated with a vector molecule to obtain transcripts, and testing of the function of the transcripts or of the proteins encoded thereby after translation of said transcripts.
机译:本发明涉及一种用于分离和表征包含核酸的生物样品中潜在存在的功能的方法,其特征在于其包括以下步骤:从所述样品开始制备核酸片段,将每个所述核酸结合在一起。具有载体分子的片段,分离与载体分子或与由与载体分子相关的片段组成的每个构建体的一部分的每个片段,体外处理与载体分子或与每个构建体的一部分相关的每个片段由与载体分子相关的片段组成以获得转录本,并在翻译所述转录本后测试所述转录本或由此编码的蛋白质的功能。

著录项

  • 公开/公告号DE69937453D1

    专利类型

  • 公开/公告日2007-12-13

    原文格式PDF

  • 申请/专利权人 PROTEUS S.A.;

    申请/专利号DE1999637453T

  • 申请日1999-08-11

  • 分类号C12N15/09;C12Q1/68;C12N1/21;C12N15;C12N15/63;

  • 国家 DE

  • 入库时间 2022-08-21 19:47:28

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