Producing a cell line useful for producing therapeutic proteins comprises integrating a unique recombinase recognition site into a zone of high transcriptional activity in the genome of a cell

摘要

Producing a cell line comprises integrating a unique recombinase recognition site into a zone of high transcriptional activity in the genome of a cell and and integrating a sequence encoding a transcription termination signal downstream of the recombinase recognition site. Independent claims are also included for: (1) cell line produced as above; (2) nucleic acid with SEQ ID NO:1 (a defined sequence of 4419 nucleotides given in the specification; (3) vector containing the nucleic acid; (4) producing a protein of interest by culturing the cell line.