Analysis method involving addition of an amino acid to the C-terminus of a peptide

摘要

A method of facilitating analysis of a peptide 10 in a mass spectrometer comprising derivatizing the C-terminus 12 of the peptide 10 with an amino acid residue 17; 18 via a reaction with a carbodiimide reagent, yielding a derivative peptide 20, ionizing the derivative peptide with a double charge, and fragmenting the ionized derivative peptide in a mass spectrometry system. Binary fragments of the ionized derivative each include a charge, thereby facilitating sequence analysis of the peptide. The added amino acid residues may be lysine or arginine residues.