How to cleave peptide bonds at the C-terminus of the peptide, and methods for determining C-terminal amino acid sequence of the peptide

摘要

Method and the decisive method of C terminal amino acid arranging the peptide of cutting off the peptide bond of C end of the peptide where as for this invention, decrease of sensitivity is little, controls the side reaction are offered. The method of cutting off the peptide bond of C end of the peptide due to this invention, being the method of cutting off the peptide bond of C end of peptide, in order to ester to convert the glutamic acid residue of the aforementioned peptide, in order to obtain C terminal deficiency peptide which C terminal amino acid residue of process and the aforementioned peptide which react with the solution of the passing halogenation carboxylic acid which possesses peptide and alcohol deficiency does one by one in the mark, features that it possesses the process which reacts with the aforementioned peptide and the alkane acid anhydride and. As for decisive method of C terminal amino acid arranging the peptide with this invention, being decisive method of C terminal amino acid arranging the peptide, in order to ester to convert the glutamic acid residue of the aforementioned peptide, in order to obtain C terminal deficiency peptide which C terminal amino acid residue of process and the aforementioned peptide which react with the solution of the passing halogenation carboxylic acid which possesses peptide and alcohol deficiency does one by one in the mark, the process which grasps the aforementioned peptide on the basis of process and measure the molecular weight of the aforementioned C terminal deficiency peptide process and the aforementioned molecular weight which react with the aforementioned peptide and the alkane acid anhydride and,It features that it possesses.