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NEW DRUG-RESISTANT RECOMBINANT SELECTIVE MARKER GENE OF FUNGUS OF GENUS ASPERGILLUS
NEW DRUG-RESISTANT RECOMBINANT SELECTIVE MARKER GENE OF FUNGUS OF GENUS ASPERGILLUS
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机译:鼠李属真菌的新抗药性重组选择性标记基因
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摘要
PROBLEM TO BE SOLVED: To provide a new useful drug-resistant marker gene in a microorganism of the genus Aspergillus.;SOLUTION: It is found that the microorganism belonging to the genus Aspergillus becomes sensitive to carboxin by culturing the microorganism in a culture medium containing acetic acid as a carbon source. Furthermore, it is predicted that a carboxin-resistant microorganism appears and the carboxin-resistant mutant gene is produced by performing a mutation-inducing treatment of the gene of the microorganism belonging to the genus Aspergillus. In this way, a gene corresponding to a succinate dehydrogenase Ip subunit gene known as a carboxin-resistant gene of a Basidiomycetes is searched from the whole base sequences of Aspergillus oryzae RIB40. As a result, it is confirmed that the mutation is caused in the gene.;COPYRIGHT: (C)2009,JPO&INPIT
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