METHOD FOR PHENYLHYDRAZONATING SACCHARIDES AND SUGAR CHAIN, METHOD FOR ANALYZING PHENYLHYDRAZONATED SACCHARIDES AND SUGAR CHAIN BY THIS METHOD, AND METHOD FOR COMPARING AND DETERMINING SACCHARIDES AND SUGAR CHAIN USING THIS METHOD

摘要

PROBLEM TO BE SOLVED: To provide a method for phenylhydrazonating saccharides and sugar chains while suppressing the dissociation of a sialic acid from a sialyl sugar chain in phenylhydrazonation reaction, and increasing a derivatization ratio to phenylhydrazonated saccharides and/or sugar chains by bring saccharides and/or sugar chains and phenylhydrazine into reaction in an acetic-acid/organic-base/H₂O solvent or an acetic-acid/organic-base solvent, and also to provide a method for analyzing saccharides and sugar chains phenylhydrazonated by the method, and a comparison and determination method using the method.;SOLUTION: Saccharides and/or sugar chains and phenylhydrazine are brought into reaction in an acetic-acid/organic-base/H₂O solvent or an acetic-acid/organic-base solvent to phenylhydrazonate the saccharides and/or the sugar chains.;COPYRIGHT: (C)2009,JPO&INPIT