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Method of purifying/concentrating sugar chains with a sugar chain-trapping molecule and method of analyzing sugar chain structure

机译:用糖链捕获分子纯化/浓缩糖链的方法和分析糖链结构的方法

摘要

The present invention provides substance which can specifically interact with sugar chains. Further, the present invention provides a method for separating, concentrating, or purifying sugar chains or a sugar chain-containing substance in a sample, comprising the steps of: a) contacting a sugar chain-trapping carrier comprising a substance which can specifically interact with sugar chains with the sample in a fluid phase under conditions that the sugar chain-trapping carrier can react with the sugar chains or sugar chain-containing substance; b) isolating a composite of the sugar chain-trapping carrier and the sugar chains or sugar chain-containing substance from the fluid phase; and c) exposing the composite to the conditions that the interaction between the sugar chain-trapping carrier and the sugar chains or sugar chain-containing substance is at least partially eliminated.
机译:本发明提供了可以与糖链特异性相互作用的物质。此外,本发明提供了一种用于分离,浓缩或纯化样品中的糖链或含糖链物质的方法,该方法包括以下步骤:a)使糖链捕获载体与包含可以与之特异性相互作用的物质接触。在捕获糖链的载体可以与糖链或含糖链的物质反应的条件下,液相中的样品带有糖链; b)从液相中分离糖链捕获载体和糖链或含糖链物质的复合物; c)使复合物暴露于至少部分消除糖链捕获载体与糖链或含糖链的物质之间的相互作用的条件。

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