MOLECULAR INTERACTION DETECTING METHOD BY FLUOROMETRIC ANALYSIS

摘要

PROBLEM TO BE SOLVED: To properly and sensitively detect interaction of biomolecules, and the like, by observing the change in the speed of motion of the biomolecules, and the like, with an unimolecular fluorometric analysis technique such as a fluorescence correlation spectroscopy (FCS), and the like, while reducing the time, labor and cost necessary until the executing inspection.;SOLUTION: The method of this invention calculates the indicator of the speed of Brownian movement of a first molecule, based on a fluorescence intensity of a reaction mixed solution that includes the fluorescent-labeled first molecule; a second molecule provided with a predetermined modified base and a third molecule specifically combined with the predetermined modified base. In such a case that the speed of the Brownian movement of the first molecule is determined to be lower than its speed, when the molecule is isolated independently, it is determined that the second molecule is bonded to the first molecule.;COPYRIGHT: (C)2009,JPO&INPIT