A New Fluorometric Method for the Determination of Oxaliplatin Based on Cucurbit[7]uril Supramolecular Interaction

摘要

This paper proposed new competitive methods for fluorescence detection of the anti-canccr drug oxaliplatin. The methods were based on the competitive reaction of palmatinc (PAL)/bcrbcrinc (BER)/coptisinc (COP) with oxaliplatin for the occupancy of cucurbit[7juril (CB[7] cavities. The results showed that the fluorcsccncc intensity of PAL, BER, and COP regularly increased upon addition of CB[7] until a certain amount of oxaliplatin was added, at which stage the fluorescence intensity of the system qucnchcd. Using the CB[7]PAL, CB[7] BER, and CB[7]—COP systems, linear ranges in the detection of oxaliplatin of0.005 1.75, 0.010-1.50，and 0.020-1.05 μg mL~(-1)with dctcction limits of 2,3, and 7 ngmL~(-1) respectively, were obtained. These results suggest that cucurbit[7]uril is a promising drug carricr for delivering and monitoring targeted oxaliplatin, with improved anti-tumour efficacy and reduced toxicity in normal tissues.