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DIAPHORASE GENE OF CLOSTRIDIUM KLUYVERI AND ITS APPLICATION

机译:克鲁维氏梭菌二甲醚基因及其应用

摘要

PROBLEM TO BE SOLVED: To obtain a new diaphorase gene and to provide a method for mass producing a diaphorase by using the gene.;SOLUTION: The diaphorase comprises (a) a protein having a specific amino acid sequence or (b) a protein having an amino acid sequence in which one-several amino acids are deleted, substituted or added in the amino acid sequence and diaphorase activity. The method for producing a diaphorase comprises a process for culturing a transformant containing a recombinant vector of the diaphorase and a process for recovering a protein having diaphorase activity from the obtained (broth) culture product.;COPYRIGHT: (C)2009,JPO&INPIT
机译:解决的问题:获得新的心肌黄酶基因并提供使用该基因大量生产心肌黄酶的方法。解决方案:心肌黄酶包含(a)具有特定氨基酸序列的蛋白质或(b)具有在氨基酸序列和心肌黄递酶活性中缺失,取代或添加了几个氨基酸的氨基酸序列。心肌黄递酶的生产方法包括培养含有心肌黄递酶的重组载体的转化体的过程和从获得的(肉汤)培养物中回收具有心肌黄递酶活性的蛋白质的过程。版权所有:(C)2009,JPO&INPIT

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