Synthetic biology and modular-type genome for metabolic engineering

摘要

The present invention provides a composition and method for configuring a modular replacement genome in the host microorganism. With that, after such a configuration, to remove or inactivate the genome of the host organism, it is possible that you have complete control over the host cell function by substitution genome. Modular replacement genome comprises a collection of nucleic acid fragments or segments from a combination of both of these or synthetic polynucleotides or organisms or more natural one. Pair includes a regulator gene and set substantially complete for performing vital functions in minimal culture conditions or predetermined such assemblies of segments constituting the replacement genome. Easy replacement, the present invention provides a modular genome with a module suitable for adding and / or deletions.