Gene transfer vector to insect and gene product production method

摘要

PROBLEM TO BE SOLVED: To obtain a gene engineering material for easily purifying an objective protein without requiring use of recombinant baculovirus, and provide a method for producing a foreign protein by using the gene engineering material.;SOLUTION: A large amount of the foreign protein is produced in silk gland cells, outside of silk gland cells or brought to produce silk by introducing an expression gene cassette prepared by fusing a DNA sequence of 5' terminal and a DNA sequence of 3' terminal of fibroin H chain gene with a foreign protein gene. By the new method, foreign protein easily purified is produced using the silk gland without using the recombinant baclovirus, and a technique for producing the foreign protein is established.;COPYRIGHT: (C)2004,JPO&NCIPI