Bitisarietans snake venom-derived trypsin inhibitor-like protein bitoran and its use as a blood coagulation factor Xa inhibitor

摘要

The present invention provides a novel Kunitz-type inhibitor protein exhibiting high potency to inhibit serine protease activity of human blood coagulation factor Xa. The Kunitz-type inhibitor protein against human blood coagulation factor Xa according to the present invention is a Kunitz-type inhibitor protein: bitoran D from Bitis arietans venom having the following amino-acid sequence I: tables id="TABLE-US-00001" num="00001" table frame="none" colsep="0" rowsep="0" tabstyle="monospace" tgroup align="left" colsep="0" rowsep="0" cols="2" colspec colname="1" colwidth="217pt" align="right"/ colspec colname="2" colwidth="0pt" align="left"/ tbody valign="top" row entry(SEQ ID NO:1)/entry entry/ /row /tbody /tgroup tgroup align="left" colsep="0" rowsep="0" cols="2" colspec colname="1" colwidth="217pt" align="left"/ colspec colname="2" colwidth="0pt" align="left"/ tbody valign="top" row entrySKKRP DFCYL PADDG PCRAF IPSFY YNSTS NECNT FIYGG/entry entry/ /row row entry /entry /row row entryCYGNA NKFES MDECR KTCVA SA,/entry /row /tbody /tgroup /table /tables a Kunitz-type inhibitor protein: bitoran V from Bitis arietans venom having the following amino-acid sequence II: tables id="TABLE-US-00002" num="00002" table frame="none" colsep="0" rowsep="0" tabstyle="monospace" tgroup align="left" colsep="0" rowsep="0" cols="2" colspec colname="1" colwidth="217pt" align="right"/ colspec colname="2" colwidth="0pt" align="left"/ tbody valign="top" row entry(SEQ ID NO:2)/entry entry/ /row /tbody /tgroup tgroup align="left" colsep="0" rowsep="0" cols="2" colspec colname="1" colwidth="217pt" align="left"/ colspec colname="2" colwidth="0pt" align="left"/ tbody valign="top" row entryC RQNRP DFCYL PAVEG PCRAY IRSFF YNSTS NECEK FFYGG/entry entry/ /row row entry /entry /row row entryCYGNA NKFET RDECR KTCVA SA,/entry /row /tbody /tgroup /table /tables or a modified protein thereof.