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(A) below revelation mannered null of the new promotor

机译:(A)在下面的启示中,提出了新启动子无效的内容

摘要

PROBLEM TO BE SOLVED: To provide a new promoter enabling the expression of a protein in a mold and containing a DNA having a specific base sequence and a DNA hybridizable with the DNA under stringent condition and capable of expressing a gene connected to the downstream.;SOLUTION: This new promoter contains a DNA having the base sequence of formula or a DNA hybridizable with the DNA having the above base sequence under stringent condition and capable of expressing a gene connected to the downstream. A desired protein can be expressed in large quantity by transducing the promoter into Acremonium cellulolyticus. The promoter can be prepared by preparing a library from a genom DNA derived from Acremonium cellulolyticus by conventional method and screening with a probe produced from a partial gene of protein having highest expression amount in the mold.;COPYRIGHT: (C)2001,JPO
机译:解决的问题:提供一种新的启动子,其能够使蛋白质在霉菌中表达,并包含具有特定碱基序列的DNA和在严格条件下可与该DNA杂交并能够表达与下游连接的基因的DNA。解决方案:该新启动子包含一个具有下式的碱基序列的DNA或可在严格条件下与具有上述碱基序列的DNA杂交的DNA,并且能够表达与下游连接的基因。通过将启动子转导至纤维素解孢霉中,可以大量表达所需蛋白质。启动子可以通过以下方法制备:通过用常规方法由溶解顶孢纤维素酶的基因组DNA制备文库,并用由在霉菌中具有最高表达量的蛋白质的部分基因产生的探针进行筛选。版权所有:(C)2001,JPO

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