Gene ta in order to introduce the imported gene to high efficiency gene tagetsuteingubekuta

摘要

In the process of producing knockout mice of ZO-1 gene, we have developed a gene targeting vector for homologous recombination to occur with a high probability of 90%. As a result of examination of the optimal conditions of electroporation on epithelial cells with the targeting vector, the optimal conditions for electroporation capable of efficient gene targeting for EpH4 mouse epithelial cell line I was successful in determining the. By using the vectors of the present invention, it is possible to easily introduce the allyl ZO-1 a foreign gene for ES cells. Also, because no need to consider the impact of genomic structure also good, that the drawback of stable transformants produced when the cells and transgenic mouse production conventional can be solved is expected.