Human neural stem cells derived from human amnion mesenchymal cells

摘要

PROBLEM TO BE SOLVED: To provide a human neural stem cell stably suppliable without causing problems in compatibility during transplantation. SOLUTION: This cell is separated from a human amniotic mesenchymal cell layer and capable of expressing nestin and vimentin which are markers of the neural stem cell and incorporating 5-bromo-2'-deoxy-uridine into the cell by culturing the cell in the presence of the 5-bromo-2'-deoxy-uridine. Thereby, the cell can be collected in a large amount together with placenta and the collection of the cell is free from ethical problems. The cell can stably be supplied. Since the cell has immunological tolerance, there is no problem about the compatibility when the cell is transplanted to a patient. Therefore, the cell exhibits excellent effects as a drug delivery system, etc., for the substance produced with a neurocyte by transplanting the cell into the brain of the patient suffering from an intractable nervous disease such as Parkinson's disease and metabolic nervous disease.