Specific oligonucleotides for the detection, identification and quantification of certain listery strains. (Machine-translation by Google Translate, not legally binding)

摘要

Specific oligonucleotides for the detection, identification and quantification of certain strains of listeria. Said oligonucleotides possess a sequence (seq id nos: 1 to 11) of nucleotides that is specific for a region of a gene of a strain of listeria selected from listeria monocytogenes, including its two serotypes i/iii and ii, and listeria innocua and, they are preferably selected from the hly and iap genes of listeria monocytogenes and lin02483 from listeria innocua. These oligonucleotides act as probes or primers against the target sequences of said genes. Said detection, identification and quantification of certain strains of listeria is carried out by standard pcr technology in any of its known variants and is characterized in that for the amplification of the region of the gene corresponding to the strain of listeria to be determined, said oligonucleotides. (Machine-translation by Google Translate, not legally binding)