Specific obligonucleotides for the detection, identification and quantification of isolates of clostridium tyrobutyricum. (Machine-translation by Google Translate, not legally binding)

摘要

Specific oligonucleotides for the detection, identification and quantification of clostridium tyrobutyricum isolates. The oligonucleotides of the present invention have a nucleotide sequence that is specific for a region of the fla gene specific for the species clostridium tyrobutyricum. Said oligonucleotides act as primers and probe, respectively, against the target sequences of said genes. The detection, identification and quantification of clostridium tyrobutyricum isolates is carried out by standard pcr technology in any of its known variants, especially by using real-time pcr using taqman probes, and is characterized in that for the amplification of the region of the fla gene of the clostridium tyrobutyricum isolate to be determined are used said oligonucleotides. (Machine-translation by Google Translate, not legally binding)