Transduction of genes in hematopoietic progenitor cells.

摘要

Process for transforming hematopoietic progenitor cells to express a protein comprising the steps of: co-culturing human hematopoietic progenitor cells with a population of human cells obtainable by isolation from bone marrow using fractionation density gradient in a solution 1.073 g / ml Percoll and comprising mesenchymal stem cells; and retrovirally transforming the human hematopoietic progenitor cells with a polynucleotide comprising exogenous genetic material encoding a protein in the presence of a population of human cells, wherein said protein is expressed.