Methods for administration of inhibitory RNA to plants and applications thereof.

摘要

A method for introduction of inhibitory RNA in the cytoplasm of plant cells, reducing or canceling said inhibitory RNA expression of a target gene in said plant cells, said method comprising: a) introducing into said plant cells a viral RNA vector comprising said inhibitory RNA or comprising a chimeric nucleic acid which when transcribed yields said inhibitory RNA, wherein said inhibitory RNA comprises a sense RNA or an antisense RNA comprising a nucleotide sequence of at least 100 nucleotides in length having at least 75% sequence identity with the nucleotide sequence of said target gene in said plant cell, and wherein said viral RNA vector comprises at least cis elements from a satellite RNA virus recognized by a replicase of a corresponding helper virus and he is capable of replicating in a plant cell only when provided externally of the functions essential required for said replication; b) introducing said corresponding helper virus into said plant cell.