Protein phosphorylation in signaling pathways receptor T cells.

摘要

A method for detecting or quantifying a signaling protein that is tyrosine phosphorylated in signaling pathways receptor T cells, said method comprising the step of using one or more of the following reagents to detect or quantify the signaling protein T cell receptors Cdk 6 only when phosphorylated at the tyrosine 13 or 24: (i) specific antibody isolated phosphorylation site that specifically binds said protein only when phosphorylated at the tyrosine 13 or 24 comprised in the sequence phosphorylation site SEQ ID nO: 60 or SEQ ID nO: 61, wherein said antibody does not bind said protein when not phosphorylated at said tyrosine; 60 or SEQ ID NO: 61, comprising tyrosine and / or (ii) a peptide labeled with a heavy isotope (AQUA peptide) for the quantification of said protein, said labeled peptide sequence phosphorylation site SEQ ID NO comprising phosphorylated 13 or 24.