PRODUCTION AND SECRETION OF PROTEINS OF BACTERIAL ORIGIN IN FILAMENTOUS FUNGI

摘要

The present invention is related to an improved production of bacterialproteins in filamentous fungus, e.g. in Trichoderma andAspergillus. The improvement is achieved by constructing expression vectors,which comprise the bacterial protein encoding DNA sequencesfused in frame with a DNA sequence encoding a filamentous fungus secretableprotein or one or more functional domains of said protein.Filamentous fungus hosts transformed with such expression vectors secrete thedesired proteins or enzymes, especially xylanases or cellulasesoriginating from bacteria or more preferably from actinomycetes into theculture medium of the host. The desired proteins or enzymescan be used directly from the culture medium after separation of host cells orrecovered and treated using down-stream processes, whichare appropriate for the respective application. Xylanases or cellulases fromactinomycetes produced using the above expression vectors aremost suitable for treating plant derived materials, e.g. in pulp and paperindustries.