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BETA-MANNANASE FROM COFFEE BERRY BORER, HYPOTHENEMUS HAMPEI, AND USES THEREOF

机译:来自咖啡浆果百老汇,汉普顿葡萄的β-甘露聚糖酶及其用途

摘要

The present invention relates to an isolated Beta-mannanase protein having an amino acid sequence which is 90% similar to the amino acid sequence of SEQ ID NO:1, as well as isolated polynucleotides encoding the Beta-mannanase protein, and isolated expression systems and host cells containing the polynucleotides. The present invention also relates to a method of recombinantly producing Beta-mannanase protein. Also disclosed is a method of degrading mannans and polysaccharides in plant material, which involves providing plant material and contacting the plant material with the Beta-mannanase protein of the present invention under conditions effective to degrade mannans and polysaccharides in the plant material.
机译:本发明涉及具有与SEQ ID NO:1的氨基酸序列90%相似的氨基酸序列的分离的β-甘露聚糖酶蛋白,以及编码所述β-甘露聚糖酶蛋白的分离的多核苷酸,以及分离的表达系统和含有多核苷酸的宿主细胞。本发明还涉及重组产生β-甘露聚糖酶蛋白的方法。还公开了降解植物材料中的甘露聚糖和多糖的方法,其包括提供植物材料并使植物材料与本发明的β-甘露聚糖酶蛋白在有效降解植物材料中的甘露聚糖和多糖的条件下接触。

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