MODIFIED MUTANT COLLAGENASE AND IT'S USE IN FAT MELTING AND IN SCAR REDUCTION

摘要

A Clostridium histolyticum collagenase CoIH (Glu451Asp) melts adipose tissue when injected into selected regions of the body. This protein product melts fat pads effectively in fat rat experiments, with very little side effects. Very little hemorrhage was observed. We also invent a new version of CoIH mutant by linking a peptide motif CKGGRAKDC-Gx (X is varying from 2 to 6 Gs) in front of CoIH (Glu451Asp) (called topical CoIH-FM), which can target to white fat vasculature. By combining with novel transdermal technology (such as Hydroxysome technology), we develop a topical protein cream that can melt fat. This product can be used as cellulite cream and for chemical liposuction. This topical CoIH-FM can also be injected into adipose tissue as a replacement for liposuction or as an adjunct method with liposuction. Since raise scar is formed by overgrowth of collagen, our topical CoIH-FM cream is shown to have application in scar reduction.