NUCLEIC ACIDS AND LIBRARIES FOR USE IN FUNCTIONAL ANALYSIS OF REGULATORY RNAS SUCH AS MICRORNAS (MIRNAS)

摘要

The invention relates to a nucleic acid comprising the following contiguous elements arranged in the 5 prime to 3 prime direction; a promoter; a selectable marker; a cloning site for receipt of a nucleic acid segment, said segment comprising a candidate miRNA target sequence; and a poly adenylation signal, said elements arranged such that a transcript directed by said promoter comprises said selectable marker, said candidate miRNA target sequence, and said poly adenylation signal in that order. Suitably the miRNA test sequence is or is derived from a 3'UTR. The invention also relates to methods for making and screeing libraries.