METHOD FOR THE MEASURE OF MOLECULAR INTERACTIONS BY MEASUREMENT OF THE LIGHT REFLECTED BY PLANAR SURFACES

摘要

Method of determining the binding constant of an interaction of a ligand with a receptor comprising: bringing a planar surface of a transparent solid material consisting of a hydroyphobic amorphous polymer having a refractive index between 1.32 and 1.35 into contact with a solution containing a receptor molecule or reagent; adding to the solution a sequence of known volumes of an aqueous solution of a ligand; measuring at each addition a light intensity value reflected from the interface between the solid material and the aqueous solution; fitting the measured values as a function of the ligand additions with the Fresnel formulas to obtain the adhered mass and with the Langmuir formula to obtain the ligand-receptor affinity constant at equilibrium.