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Method for measuring molecular interactions by measurement of light reflected by planar surfaces
Method for measuring molecular interactions by measurement of light reflected by planar surfaces
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机译:通过测量由平面反射的光来测量分子相互作用的方法
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摘要
A procedure for the quantitative determination of interactions of ligands with receptors adsorbed or immobilized on the surface of a solid material which can be functionalized, transparent and with low refractive index, by direct measure of the reflection of light. The procedure includes utilizing a planar surface, flat or rough, of a transparent solid material formed from a hydrophobic amorphous polymer, having a refractive index between 1.3200 and 1.3500, which is brought into a solution of a mixture containing from 1 nanogram/ml to 10 milligrams/ml in concentration of molecules with a function of receptor or reagent.
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