A METHOD OF PRODUCING GLUCAGON-LIKE PEPTIDE 1(GLP-1)7-36 AND AN GLP-1 ANALOGUE

摘要

The present invention discloses a method of producing insulinotropic GLP-1 (7-36) polypeptide and/or GLP-1 analogs by ligating genes in a tandem way. Also disclosed are the recombinant polypeptides produced by such a method. Using the method of this invention, 1 to 32 copies of GLP-1(7-36) and/or GLP-1 analog genes may be expressed in tandem. The desired polypeptide can be obtained after cleavage of a fusion protein and further processes of separation and purification. This invention makes it possible to produce the recombinant GLP-1(7-36) and/or GLP-1 analogs on a large scale, at a significantly reduced production cost.