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A method of producing glucagon-like peptide 1(GLP-1)7-36 and an GLP-1 analogue
A method of producing glucagon-like peptide 1(GLP-1)7-36 and an GLP-1 analogue
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机译:一种胰高血糖素样肽1(GLP-1)7-36和GLP-1类似物的生产方法
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摘要
A genetically engineered bacterium for preparing the peptide GLP-1(7-36) promoting insulinotropic hormone secretion uses colibracillus as its host cell, which carries the plasmids containing 1-32 genes able to express GLP-1 (7-36). A genetically engineered bacterium for preparing the peptide GLP-1(7-36) promoting insulinotropic hormone secretion uses colibracillus as its host cell, which carries the plasmids containing 1-32 genes able to express GLP-1 (7-36). A process for configuring the bacterium includes cloning the 1-32 gene to colibacillus, transferring the plasmid to colibracillus, fermenting culture in liquid culture medium to generate and collect the inclusion body containing GLP-1(7-36), cracking fusion protein, separating and purifying. Its advantages include low cost.
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