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METHOD FOR INVESTIGATING CYTOSINE METHYLATIONS IN DNA
METHOD FOR INVESTIGATING CYTOSINE METHYLATIONS IN DNA
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机译:研究DNA中胞嘧啶甲基化的方法
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摘要
The invention relates to a method for sensitively and specifically detecting cytosine methylations. For this purpose, DNA is first analysed by reacting with the aid of a methylation specific restriction enzyme. In such a way, the background DNA is removed from a reaction preparation. At a next step, a specific conversion of a non-methylated cytosine is carried out, while a methylated cytosine remains unchanged. The converted DNA can be analysed according to different methods, in particular by means of real time PCR method.
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