Immunoassay and molecular methods to investigate DNA methylation changes in peripheral blood mononuclear cells in HIV infected patients on cART

摘要

This study aimed to investigate the influence of antiretroviral therapy on methylation markers, in a group of HIV infected, heavily treated patients. Immune and molecular methods were used to investigate potential changes in methylation profile in DNA isolated from peripheral blood mononuclear cells collected from antiretroviral-experienced HIV infected patients and healthy controls. The percentage of 5-methylcytosine was inversely correlated with proviral DNA and active replication while DNMT1 (p=0.01) and DNMT3A (p=0.004) independently correlated with active viral replication. DNMT3A expression increased with total treatment duration (p=0.03), number of antiretroviral drugs ever used (p=0.003) and cumulative exposure to protease inhibitors (p=0.02) even in currently HIV undetectable patients.