METHOD FOR THE COUPLED ENZYME IMMUNOCHEMICAL ASSAY OF ANALYTES BY MEANS OF ENDOGENOUS CALIBRATORS

摘要

The invention relates to a method for assaying a plurality of analytes such as e.g. metabolites and antigens in biological and other liquid samples, by means of analytical elements, especially lateral flow test strips, flow-trough membrane systems (flow-through tests), wells/cavities of microtiter plates or test tubes. The method according to the invention is based on coupled enzyme and affinity reactions and is carried out by means of endogenous calibrators, i.e. endogenously produced substances, by means of which dilutions of sample matrices can be corrected (e.g. creatinine, glucose, glucose-6-phosphate, lactate, glutamate, aspartate, cholesterol, pyruvate, urea and triglycerides). The invention can especially be applied in medical diagnostics, in the pharmaceutical industry and in environmental protection. The invention preferably relates to the simultaneous or sequential assay of antigens and metabolites. According to the invention, these are high-molecular antigens, such as e.g. proteins, or low-molecular haptens, such as e.g. pesticides, neopterin, harmful substances or hormones, the metabolites are e.g. glucose or creatinine. The results can be directly inferred from the assay by means of a nomogram, a comparator (reference strip), a reader or by mere visual comparison.