Gene mapping method using microsatellite genetic polymorphism markers

摘要

A gene mapping method comprises collecting a DNA sample from to-be-screened subjects and control subjects; using forward primers having a length of 15 to 100 nucleotides and having the same nucleotide sequences as the sequence extending in 3'-direction from the 5'-terminus of each of the DNA sequences referenced with SEQ ID No: 1 to 27088 and reverse primers having a length of 15 to 100 nucleotides and having a nucleotide sequence complementary to the sequence extending in 5'-direction from the 3'-terminus of each of the DNA sequences referenced with SEQ ID No: 1 to 27088 to develop a polymerase chain reaction on the obtained DNA sample, and providing DNA sequence fragments including microsatellite genetic polymorphism markers; and statistically comparing the DNA sequence fragments obtained from the to-be-screened subjects with those obtained from the control subjects so as to identify an existing region of a pathogenic gene or a gene relating to human phenotypes with genetic factors.