KNOCK-IN MOUSE FOR MODELLING BLOCKADE OF HUMAN TNFALPHA

摘要

The invention relates to a transgenic mouse, wherein the complete endogenous murine TNFα-gene including its upstream regulatory sequences is replaced by the human homologous TNFα-gene including its upstream regulatory sequences or a mutant thereof, wherein the mutant is characterized by deletion, addition, substitution, and/or inversion of one or more nucleotides so that the functional properties of the modified human TNFα-gene are conserved and cells, tissues and cell lines derived thereof. The invention further relates to an in-vivo method of screening, investigating and/or preclinical testing of compounds and/or drug candidates for human anti-TNFα therapy using said transgenic mouse and an analogous method using the cells, tissues and cell lines derived thereof.