ASSESSING BIOFILM FORMATION ONTO NATURAL SOIL PARTICLES

摘要

A method of measuring a content of a biofilm formed on a surface of soil particles is provided to measure content of a biofilm formed on a surface of soil particles accurately, rapidly and simply by measuring the biofilm formed on a surface of soil particles quantitatively with an interference of the soil. Soil for measuring a content of a biofilm is put into sterilized petri dish. The movable microorganism except for the biofilm formed in the soil particles is cleaned away by precipitating the soil by injecting distilled water in the petri dish. The washed soil is dried at 28 - 32°C for 25 - 35. The biofilm formed in the soil particles is dyed by putting a crystal violet solution into the dried soil passing through the drying step. The crystal violet solution which dyed remains in the biofilm formed in the soil particles is removed. The crystal violet dyed in biofilm turns is decolorized. An absorbance of a solution passing through the decoloration step is measured.