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DNA CHIP, KIT FOR DETECTING GENOTYPE OF HUMAN PAPILLOMAVIRUS AND DETECTION METHOD USING THE SAME

机译:用于检测人乳头瘤病毒基因型的DNA芯片,试剂盒及其检测方法

摘要

A DNA chip and a kit for detecting a plurality of genotypes of human papillomavirus(HPV) at a time through PCR are provided to improve inspection specificity to 36 sorts of HPV genotypes and rapidly inspect superinfection of HPV. A DNA chip for detecting a plurality of genotypes of human papillomavirus(HPV) at a time through PCR(polymerase chain reaction) contains one or more probes among probes having sequences from SEQ ID NO:5 to SEQ ID NO:40. A kit for detecting a plurality of HPV genotypes at a time through PCR comprises a DNA extraction reagent, a PCR reagent and one or more probes among probes having sequences from SEQ ID NO:5 to SEQ ID NO:40. A method for detecting HPV genotypes consists of the following steps of: extracting DNA from an analytic cell; amplifying the DNA through the PCR; hybridizing the amplified DNA to an HPV bio-chip; and confirming the specifically coupled HPV genotype in the hybridization.
机译:提供一种通过PCR一次检测多种人乳头瘤病毒(HPV)基因型的DNA芯片和试剂盒,以提高对36种HPV基因型的检查特异性,并快速检查HPV的重复感染。用于通过PCR(聚合酶链反应)一次检测多种人乳头瘤病毒(HPV)基因型的DNA芯片在具有SEQ ID NO:5至SEQ ID NO:40的序列的探针中包含一个或多个探针。一种用于通过PCR一次检测多种HPV基因型的试剂盒,其包含DNA提取试剂,PCR试剂和具有SEQ ID NO:5至SEQ ID NO:40的序列的探针中的一个或多个探针。检测HPV基因型的方法包括以下步骤:从分析细胞中提取DNA;通过PCR扩增DNA;将扩增的DNA与HPV生物芯片杂交;并确定杂交中特异性偶联的HPV基因型。

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