Comparison of Human Papillomavirus Detection and Genotyping with Four Different Prime Sets by PCR-Sequencing

摘要

Objective To assess and compare the Human Papillomavirus(HPV) detection efficiency and the potential clinical utility of PCR sequencing‐based technology.Methods Four HPV consensus primer sets(GP5+/6+,MGP,MY09/11,and PGMY09/11) were used in order to amplify a broad spectrum of HPV types for HPV infection in 325 cervical samples and the PCR products were sequenced afterwards for the HPV genotyping.Results The HPV‐positive rate was 75.4%,of which 35.5% harbored more than one HPV genotype.A total of 36 different genotypes was found,with HPV 16(24.1%) being the most prevalent,followed by HPV 58(13.3%) and HPV 52(9.6%).There were substantial to almost perfect agreements between different primer sets regarding HPV detection efficiency,with the kappa value varying from 0.751 to 0.925,MGP,and PGMY09/11 were the most effective in detecting multiple infections(P<0.001).With each of the primer sets,a board range of HPV types could be identified,though there were several differences for a few genotypes.Conclusion The substantial agreement between PCR‐sequencing and HC2 for the detection of high‐risk HPV(kappa=0.761) indicated that PCR‐sequencing is also suitable for routine HPV screening.