PEDIOCOCCUS PENTOSACEUS ZEN1 HAVING ANTIBACTERIAL ACTIVITY AND PROBIOTICS COMPRISING PEDIOCOCCUS PENTOSACEUS ZEN1

摘要

A lactic acid bacteria originating from a fish intestines is configured to ensure that lactic acid, survivability, and the movable high production rate of antibiotic as probiotics. One Pediococcus pentosaceus abdicates 1 (KAC 91336P) and is isolated with an intestines for fish and has antibacterial activity. A kind of method includes: to extract stomach and internal organs and the step for executing shaking flask culture for Pediococcus pentosaceus buddhist 1 to be isolated, and 0.8% salting liquid is hung; It travels to MRS agar mediums and cultivates 48 hours steps for obtaining cluster at 30 degrees Celsius; Containing 0.The step and screening cluster that cluster is cultivated on the MRS agar mediums of 01% bromocresol green, have dead zone; A step of single colony is isolated; To pathogenic microorganisms inoculation including a step of vibrios anguillarum, Edwardsiella tarta, streptococcus be special and Staphylicoccus is especially on the MRS media containing 1% NaCl; In the selected lactic acid bacteria of 30 degrees Celsius of cultures and a step of one cluster of selection, with dead zone to 4 kinds of pathogenic microorganisms.