TRANSGENIC PIG EXPRESSING HLA-G AND DAF AND THE METHOD OF PRODUCING THEREOF

摘要

A kind of method expresses HLA-G genes, DAF genes are arranged to suppress the immunological rejection of natural killer cells (natural killer cells) and use for unit theraphy and xenotransplant for producing transgene pig. A kind of method expresses human leucocyte antigen (HLA)-G (HLA-G) gene, decay-accelerator coefficient (DAF) gene includes: the step that a body cell is isolated from the clonal pigs of HLA-G for producing transgene pig clone clon line; Induce the step that the expression vector containing DAF genes enters body cell; One step of the screening body cell that wherein expression vector is induced. Expression vector is the pCX-EGFP-BSD as a cloning vector. A kind of method expresses HLA-G genes, DAF genes include: the one-step cloning body cell system for cultivating transgene pig for producing transgene pig; Remove a core of ovum and the one-step cloning body cell of fusing; Ovum is transplanted to a step for surrogate mother pig.